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. Author manuscript; available in PMC: 2014 Apr 8.
Published in final edited form as: Thromb Haemost. 2013 Feb 7;109(4):614–624. doi: 10.1160/TH12-10-0760

Figure 9.

Figure 9

Cytoprotective signaling activity of protein C derivatives in the permeability assay. (A) Confluent endothelial cell monolayers were treated with increasing concentrations of the zymogen protein C derivatives for 30 min followed by incubation of the cells with 1 nM thrombin. The permeability was induced with 10 nM thrombin (Th) for 10 min and the inhibition of cell permeability was monitored from the flux of Evans blue-bound albumin across endothelial cells as described under “Materials and methods”. Cont (−) represents negative control not incubated with thrombin. (B) The plot of maximal protection (inhibition of permeability) as a function of different concentrations of protein C derivatives derived from the data in panel A. The symbols in panel B are: wild-type protein C (○), protein C R147W (●), protein C K150del (□). Data are derived from two independent measurements (±SD).