Table 2.

Non-SET domain protein methyltransferases and their established substrates in the yeast Saccharomyces cerevisiae.

Protein (unless otherwise indicated, all are seven-beta strand methyltransferases)	Substrate and position (given from the mature N-terminal residue, unless otherwise designated)	Product(s)	Methylated residue surface exposed or buried	References
Protein lysine methyltransferases
Dot1	histone H3 Lys-79	mono, di, and trimethyl	surface	[17]
See1	elongation factor eEF1A (Tef1/Tef2) Lys-316	dimethyl	surface	[34]
Efm2	elongation factor EF3A (Yef3) Lys-186	trimethyl	unknown	[35]
Rkm5	ribosomal protein Rpl1 Lys-46	monomethyl	surface	[18]
Protein arginine methyltransferases
Rmt1 (Hmt1)	many	omega-monomethyl; omega-asymmetric dimethyl		[38]
Rmt2	ribosomal protein Rpl12ab Arg-66	delta-monomethyl	protein surface	[39]
Hsl7	unknown	omega-monomethyl; omega-symmetric dimethyl		[40]
Sfm1 (SPOUT family methyltransferase)	ribosomal protein Rps3 Arg-145	omega-monomethyl	buried - close contacts with 18S rRNA adenine-1427	[8]
N-terminal protein methyltransferase
Nmt1	ribosomal protein Rpl12ab Pro-1 ribosomal protein Rps25ab Pro-1 several others?	N-dimethyl N-dimethy1	surface surface	[12]
Protein histidine methyltransferase
Hpm1	Ribosomal protein Rpl3 His-242	3-(tau)methyl	buried - contacts with 25S rRNA O6 guranine-878 and OP1 adenine-876	[14]
Protein glutamine methyltransferases
Mtq1	mitochondrial translational release factor Mrf1 Gln-287 (from initiator Met)	N-5-monomethyl amide	surface	[41]
Mtq2	cytoplasmic translational release factor Sup45 Gln-182	N-5-monomethyl amide	surface	[41]
C-terminal protein methyltransferase
Ppm1	Protein phosphatase 2A catalytic subunit C-terminal Leu	methyl ester	surface	[42]
C-terminal protein isoprenylcysteine methyltransferase
Ste14 (membrane-bound methyltransferase)	Many	C-terminal isoprenylated cysteine methyl ester	surface	[43]