Fig. 2.

CTX- and IL-2-induced Treg modulation. Patient PBMCs were collected at different time points during the vaccination (a, c) and observation (b, d) periods (see the Online figure 1). The PBMCs were stained with anti-CD4, anti-CD25, and anti-Foxp3 mAbs and were analyzed by FACS. The graphs show the CD4⁺CD25^hiFoxp3⁺ Treg frequency (Tregs) in the gated CD4⁺ T subset (a, b) and the frequency of CD4⁺ cells in the gated lymphocytes (c, d) (the p values were calculated using Wilcoxon statistical tests)