Figure 2.

Cellular pathways induced by miR-221. The Cignal Finder 10-Pathway Reporter Arrays (SABiosciences) was employed. Relative luciferase activity was measured in SNU-398 (low miR-221 expression) or in SNU-398/miR221 clone 2, which shows a miR-221 expression about 2.5-fold higher than in SNU-398 cells (see Figure S1). Each condition was measured in triplicate. A t-test was used to compare the average expression of each condition in the two cell lines. Wnt, cell cycle, NFkB, and Myc exhibited a significant increased activity in the SNU-398/miR221 cells. ^*p-value ≤ 0.05; ^**p-value ≤ 0.01; ^***p-value ≤ 0.001.