Table 2.
Genes individually validated as miR-221 targets.
| Gene | Gene symbol | Sylamer positiona | 3′UTR length (bp) | Number of miR-221 target sequences | Fold-changeb | Online predictionc |
|---|---|---|---|---|---|---|
| Retinoblastoma 1 | RB1 | 1087 | 1915 | 2 | −1, 2 | |
| Apoptotic Peptidase Activating Factor 1 | APAF1 | 430 | 2945 | 2 | −1, 3 | |
| Annexin A1 | ANXA1 | 1580 | 718 | 2 | −1, 16 | M |
| WEE1 homolog (S. Pombe) | WEE1 | 1598 | 1382 | 1 | −1, 16 | |
| CCCTC-binding factor (zinc finger protein) | CTCF | 302 | 1308 | 2 | −1, 37 | D |
| Fas ligand (TNF superfamily, member 6) | FASLG | N/A | 907 | 1 | N/A |
a
Position within the first 1800 most down-regulated mRNAs.
b
Calculated on the basis of microarray experiments (3 miR-221 vs. 4 NC2).
c
M, Microcosm; D, Diana microT; T, Targetscan.