Figure 4.
Affymetrix GeneChip analysis of CNS cytokine gene expression in brains from Pb-treated and control female mouse pups. Pb-treated female mouse pups were exposed from gd8-pd21 to 0.1 mM Pb acetate via the dam’s drinking water. RNA was isolated, using the Qiagen Lipid Tissue Midi kit, from brain tissue pooled within each litter; 3 litters per treatment. The GeneChip used for the analysis was MG 430A from Affymetrix. Cytokine RNA expression was normalized to endogenous expression levels for GAPDH and results represent mean ± SD.