Blue arrows point to maximum shifts produced by the biochar amendment, i.e. a decrease in the total cumulative N2O, the N2O/(N2 + N2O) ratio, and the flux of total N denitrified (N2 + N2O). Eigenvalues of the first two axes are 0.343 and 0.161, the sum of all canonical axes is 0.555. “Cumulative N2O” represents the difference (control-biochar) in total N2O emitted during the entire incubation period; “ratio” and “Total N denitrified” represent the differences (control-biochar) at the day selected for isotopic gas analysis (see Fig. S1). Tsilt, Tclay and Tsand represent the percentages of soil silt, clay and sand. DOC: dissolved organic C in soil.