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. Author manuscript; available in PMC: 2013 Apr 26.
Published in final edited form as: Mol Cell. 2012 Sep 6;48(2):254–265. doi: 10.1016/j.molcel.2012.08.002

Figure 2. Physical Detection of GAA/TTC-Induced DSBs.

Figure 2

Location of GAA/TTC tracts on chromosome V is depicted. Intact chromosome V is ~585 kb, DSB at the tract results in a 43 kb broken fragment. Chromosomal DNA was separated by CHEF. Both intact and broken chromosomal V were detected by southern blot hybridization using a HPA3-specific probe. Lanes: 1, wild-type strain with (GAA)20; 2, wild-type strain with (GAA)230; 3, TET-TAF4 strain with (GAA)230; 4, TET-RFA2 strain with (GAA)230.