Table 1.
Effect of Mutants Identified in the Genome-wide Screen on GAA-Induced GCRs
| Genetic Background | Rate of GCR (×10−7) | |||||
|---|---|---|---|---|---|---|
| (GAA)0 | (GAA)120 | (GAA)230 | ||||
| Wild-type | 0.03 (0.02–0.04)a | 3 (3–4) | 20 (10–30) | |||
| Double-strand break repair genes | ||||||
| Δmre11 | 36 (27–42) | 1,200b | 126 (110–140) | 42 | 350 (240–400) | 18 |
| Δsae2 | 0.9 (0.2–1) | 30 | 17 (13–24) | 6 | 60 (50–70) | 3 |
| Replication genes | ||||||
| TET-RFA2 | 4 (3–6) | 133 | 272 (93–380) | 91 | 740 (490–910) | 37 |
| TET-POL12 | 4 (1–8) | 133 | 65 (46–72) | 22 | 280 (70–580) | 14 |
| TET-PRI2 | 1 (1–2) | 33 | 49 (39–51) | 16 | 630 (550–910) | 32 |
| TET-POL3 | 0.5 (0.2–0.7) | 17 | 30 (23–40) | 10 | 150 (140–200) | 8 |
| TET-POL2 | 0.2 (0.2–0.5) | 7 | 7 (5–8) | 2 | 80 (70–100) | 4 |
| TET-POL30 | 0.6 (0.4–1) | 20 | 32 (21–40) | 11 | 370 (290–540) | 19 |
| TET-RFC2 | 0.2 (0.1–0.4) | 7 | 30 (9–41) | 10 | 280 (250–860) | 14 |
| TET-DNA2 | 0.5 (0.3–0.7) | 17 | 16 (12–21) | 5 | 170 (150–200) | 9 |
| TET-MCM4 | 2 (1–3) | 67 | 28 (20–31) | 9 | 150 (90–210) | 8 |
| TET-ORC4 | 3 (2–4) | 100 | 26 (19–33) | 9 | 160 (130–220) | 8 |
| Δrad27 | 12 (7–20) | 400 | 32 (26–46) | 11 | ND | ND |
| Δrtt101 | 3 (2–3) | 100 | 15 (12–16) | 5 | 140 (120–360) | 7 |
| Δmms1 | 0.14 (0.1–0.4) | 5 | 16 (12–21) | 5 | 200 (120–830) | 10 |
| Δmrc1 | 4 (3–8) | 133 | 58 (45–66) | 19 | 290 (210–350) | 15 |
| Δtof1 | 2 (0.9–3) | 67 | 35 (29–53) | 12 | 210 (170–230) | 11 |
| Telomere maintenance genes | ||||||
| TET-TEN1 | 0.2 (0.2–0.5) | 7 | 16 (5–28) | 5 | 160 (90–300) | 8 |
| TET-CDC13 | 2 (0.8–2) | 67 | 13 (10–18) | 4 | 220 (180–260) | 11 |
| Transcription initiation genes | ||||||
| TET-TAF4 | 0.2 (0.07–0.2) | 7 | 132 (105–170) | 44 | 460 (390–660) | 23 |
| TET-TAF9 | 0.1 (0.05–0.2) | 3 | 23 (9–31) | 8 | 190 (130–370) | 10 |
| TET-TOA1 | 0.6 (0.4–1) | 20 | 36 (17–44) | 12 | 210 (200–270) | 11 |
| TET-SUA7 | 0.2 (0.1–0.4) | 7 | 86 (60–120) | 29 | 390 (370–460) | 20 |
| TET-TFG1 | 0.5 (0.2–1) | 17 | 23 (17–27) | 8 | 370 (310–450) | 19 |
| TET-SPN1 | 0.3 (0.2–0.4) | 10 | 10 (7–11) | 3 | 160 (100–240) | 8 |
ND, not determined. See also Tables S1, S2, and S3.
a
Numbers in parentheses correspond to the 95% confidence intervals.
b
Fold increase in GCR rates in mutants compared to wild-type strains.