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. Author manuscript; available in PMC: 2013 Oct 4.
Published in final edited form as: Nature. 2013 Apr 4;496(7443):110–113. doi: 10.1038/nature12038

Figure 1.

Figure 1

Sirt6 prefers to hydrolyze long chain fatty acyl lysine in vitro. (A) HPLC traces showing Sirt6-catalyzed hydrolysis of different acyl peptides based on the H3K9 sequence. (B) H2B K12 myristoyl peptide can be hydrolyzed by Sirt6 while the corresponding acetyl peptide cannot. Reactions were carried out with 50 μM peptide, 1 μM Sirt6, 20 mM Tris pH 8.0, 0.5 mM NAD, and 1 mM DTT at 37°C for 30 min.