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. Author manuscript; available in PMC: 2013 Oct 4.
Published in final edited form as: Nature. 2013 Apr 4;496(7443):110–113. doi: 10.1038/nature12038

Figure 3.

Figure 3

Sirt6 regulates TNFα fatty acylation and secretion. (A) Method of using Alk14 to detect TNFα fatty acylation. (B) Sirt6 controls TNFα fatty acylation on K19 and K20. (C) H133 of Sirt6 is required for TNFα defatty-acylation. (D) Sirt6 defatty-acylates TNFα in vitro. (E) Sirt6 regulates secretion of TNFα in MEF cells. n = 6; ***P < 0.001. (F-I) Sirt6 regulates fatty acylation level and secretion of endogenous TNFα in THP-1 cells (F and G; n = 3; **P < 0.01) and bone marrow-derived macrophages (H and I; n = 5; *P < 0.02). Secretion data were expressed as mean ± sd.