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. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: Dev Biol. 2012 May 1;367(2):114–125. doi: 10.1016/j.ydbio.2012.04.027

Figure 1. raw mutants exhibit ensheathment defects.

Figure 1

(A,B) Stage 15 gonads stained to examine germ cell ensheathment. (A, A′) 68-77/+. (B, B′) raw134.47, 68-77/raw155.27, 68-77. Embryos are immunostained for VASA (blue, germ cells), 68-77-lacZ (red, SGPs), and Neurotactin (NRT) (green, SGP cell surface). (A′, B′) NRT alone. (C, D) Stage 12 gonads stained to examine EYA expression as an indicator of SGP specification. SGPs are outlined with a white dashed line. (C) Control gonad: 68-77/68-77. (D) raw134.47, 68-77/raw155.27, 68-77 gonad. (E, F) Expression of TJ in stage 15 embryos to assess SGP identity. Traffic jam (green, SGPs) and VASA (blue, germ cells). (E) Control gonad: raw134.47, 68-77/+ or raw155.27, 68-77/+. (F) raw134.47, 68-77/raw155.27, 68-77 gonad. Scale bar: 10 μm for all immunofluorescence images. Posterior right.