Figure 6. raw regulates germline-soma interactions through cadherin-based adhesion.

(A) Quantitation of ensheathment defects using anti-ß-gal immunostaining of 68-77-lacZ. Note that loss of DE-cadherin (shotgun) enhances the ensheathment defects observed in raw mutants while increasing DE-cadherin expression with tub-DE-cad rescues these defects. Genotypes: raw/+ (raw^134.47, 68-77/+ or raw^155.27, 68-77/+); raw/raw (raw^134.47, 68-77/raw^155.27, 68-77); raw/raw, DE-cad (raw^134.47, shg^g317, 68-77/raw^155.27, 68-77); raw/raw, DE-cad/DE-cad (raw^134.47, shg^g317, 68-77/raw^134.47, shg^g317, 68-77); DE-cad/DE-cad (shg^g317, 68-77/shg^g317, 68-77); DE-cad/+ (shg^g317, 68-77/+) ; raw/+; tub-DE-cad (raw¹⁵⁵²⁷, 68-77/+; tub-DE-cad^WT) ; raw/raw; tub-DE-cad (raw^134.47, 68-77/raw^155.27, 68-77; tub-DE-cad^WT). (B) Western blot analysis of DE-cad protein levels in raw mutants (raw^134.47/raw^155.27) relative to controls. (C, D) DE-Cad expression in stage 15 embryos. VASA (blue, germ cells), 68-77-lacZ (red, SGPs), DE-cad (green). (C′, D′) DE-cad alone. (C, C′) raw heterozygous control, (D, D′) raw^134.47/raw^155.27. (E, F) ARM expression in stage 15 embryos. VASA (blue, germ cells), 68-77-lacZ (red, SGPs), ARM (green). (E′, F′) ARM alone. (E, E′) raw heterozygous control, (F, F′) raw^134.47/raw^155.27. (G) ARM levels at sites of SGP-GC contact in raw/+ (raw^134.47, 68-77/+ or raw^155.27, 68-77/+), and raw/raw (raw^134.47, 68-77/raw^155.27, 68-77) with or without ensheathment defects in stage 14/15 embryos. (H) Western blot analysis of phospho-ARM protein levels relative to total ARM in raw mutants (raw^134.47raw^155.27) relative to controls (Oregon R). (I, J) ARM expression in stage 15 embryos overexpressing DE-cad. VASA (blue, germ cells), 68-77-lacZ (red, SGPs), ARM (green). (I′, J′) ARM alone. (I, I′) raw/+; tub-DE-cad (raw^155.27or raw^134.47, 68-77/+;tub-DE-cad^WT). (J, J′) raw/raw; tub-DE-cad (raw^134.47, 68-77/raw^155.27, 68-77; tub-DE-cad^WT). Scale bars: 10 μm. Posterior right.