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PEGylation by post-insertion of HDAS-PEG attenuates liposome-induced complement activation. The complement proteins in human serum were determined by enzyme-immunoassay after treatment with plain liposomes or HDAS-PEG liposomes for 1 h. C4d and Bb are the markers for classical and alternate pathways, respectively, whereas SC5b is S protein-bound terminal complex. Average titers and standard error of mean were determined for six individual reading (* p < 0.05).
