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. 2012 Dec 20;7(5):1051–1064. doi: 10.1038/ismej.2012.167

Table 1. Description of analyzed soils, including sampling period, and assignment to conducted analyses.

Soil Soil typea Location Vegetationb (land use intensity)c pHd NO3/NH4+ (μg gTG−1) Samplede Analysis
FG Cambisol N50°08′ E11°52′ Grassland (ni) 6.0 2010 MeOH dis
OG Histosol N49°57′37′′ E11°35′42′′ Grassland (ni) 7.0 2010 MeOH dis
AEG 2 Leptosol N48° 22′ 36.686′′ E9° 28′ 22.023′′ Grassland (i) 6.9 2.8/23.8   Pyro Isol MPN
AEG 7 Leptosol N48° 23′ 29.116′′ E9° 22′ 36.65′′ Grassland (ni) 7.6 1.8/23.2   Pyro MPN
AEW 5 Cambisol N48° 25′ 10.626′′ E9° 24′ 52.854′′ Forest (i) 5.6 1.3/24.7   Pyro MPN
AEW 8 Cambisol N48° 22′ 57.322′′ E9° 22′ 56.584′′ Forest (ni) 6.4 2.2/25.0   Pyrof Isol MPN
HEG 6 Stagnosol N51° 12′ 53.766′′ E10° 23′ 28.395′′ Grassland (i) 6.5 0.4/20.2   MeOH dis Pyro TRFLP Isol MPN
HEG 9 Stagnosol N51° 13′ 26.031′′ E10° 22′ 50.834′′ Grassland (ni) 7.0 0.3/19.7 2008 2009 Pyro Isol MPN
HEW 5 Luvisol N51° 15′ 49.961′′ E10° 14′ 27.448′′ Forest (i) 5.4 1.0/25.0   Pyro Isol MPN
HEW 12 Luvisol N51° 6′ 2.477′′ E 10° 27' 18.659” Forest (ni) 4.8 0.6/20.9   Pyro Isol MPN
SEG 2 Histosol N53° 5′ 21.505′′ E13° 58′ 48.169′′ Grassland (i) 7.5 1.6/24.9   Pyro
SEG 6 Histosol N53° 6′ 12.583′′ E13° 37′ 22.2′′ Grassland (ni) 5.8 1.6/23.7   Pyro MPN
SEW 5 Cambisol N53° 3′ 25.321′′ E 13° 53′ 7.318′′ Forest (i) 4.0 0.6/16.4   Pyrog,f,h Isol MPN
SEW 9 Cambisol N53° 2′ 40.513′′ E13° 48′ 36.371′′ Forest (ni) 4.5 0.4/15.2   Pyrog Isol MPN

Abbreviations: Isol, isolation of pure cultures; MeOH dis, determination of methanol dissimilation based on the amount of 14CO2 produced from 14C-methanol; MPN, most probable numbers of methanol-utilizing methylotrophs (details in Supplementary Information ‘Methods'); Pyro, pyrosequencing of genes mxaF, fae and mch; TRFLP, terminal restriction fragment length polymorphism analysis with mch.

a

Data were taken from Fischer et al., 2010.

b

Grassland, temperate grassland with various grasses and weeds; Forest, mixed hardwood forests that were dominated by beech (Fagus sylvatica L.).

c

i, intense land use (i.e., grasslands were regularly fertilized and forests were regularly harvested); ni, non-intense land use (i.e., grasslands no fertilized and forests were not subjected to land use since at least 60 years).

d

pH in water; mean values from three independent measurements.

e

Detailed sampling scheme is given in Supplementary Table S1.

f

In these soils amplification of mch was not successful.

g

In these soils amplification of mxaF was not successful.

h

In these soils amplification of fae was not successful.