Skip to main content
. 2012 Dec 20;7(5):908–921. doi: 10.1038/ismej.2012.163

Figure 2.

Figure 2

Phylogenetic tree based on partial-derived PmoA sequences of Proteobacteria (165 amino acids). The tree was constructed via Neighbor-joining with 10 000 bootstraps. Sequences detected in surface tailings water via PCR analysis or in the metagenomic assembly from the heavy DNA fraction of a SIP experiment are indicated in bold. Major known clusters of PmoA and AmoA are indicated at the right. The scale bar represents 0.1 change per position. Bootstrap values >80% are indicated. The predominant Methylocaldum sp. OTU is in a box. The PxmA group represents a second copy of a PmoA-like sequence of unknown function recently found in the genomes of some Gammaproteobacteria methanotrophs. The tree was rooted against a set of eight AmoA sequences from Betaproteobacteria.