Table 1. Average (±s.e.m.) absolute (mℳ) SCFA levels in the luminal content of M-SHIME units during the first 3 days (18 h, 42 h and 68 h) after inoculation with faecal samples of five different human donors (n=5).
| Time after inoculation | 0 h | 18 h | 42 h | 68 h |
|---|---|---|---|---|
| Absolute values (mℳ) | ||||
| Acetate | 7.7±1.5 | 36.5±1.4 | 31.1±1.1 | 31.3±0.5 |
| Propionate | 2.5±0.4 | 8.3±0.7 | 7.0±0.4 | 7.1±0.2 |
| Butyrate | 2.9±1.1 | 7.8±1.7 | 11.3±1.1 | 9.9±0.7 |
| Valerate | 0.6±0.1 | 1.9±1.1 | 2.4±0.9 | 1.6±0.6 |
| Caproate | 0.5±0.2 | 0.3±0.3 | 0.5±0.5 | 0.3±0.3 |
| Branched SCFA | 1.2±0.1 | 3.0±0.7 | 3.7±0.9 | 2.5±0.7 |
| Total SCFA | 15.4±3.2 | 57.9±3.7 | 56.0±3.2 | 52.9±1.7 |
| Proportional values (mol%) | ||||
| Acetate | 50.8±1.2 | 63.6±2.2 | 56.0±2.4 | 59.5±2.1 |
| Propionate | 16.6±0.8 | 14.3±0.8 | 12.6±0.4 | 13.5±0.5 |
| Butyrate | 17.1±2.4 | 13.3±2.6 | 20.1±1.2 | 18.8±1.2 |
| Valerate | 4.0±0.4 | 3.0±1.7 | 4.1±1.4 | 2.9±1.1 |
| Caproate | 2.7±0.8 | 0.4±0.4 | 0.8±0.8 | 0.6±0.6 |
| Branched SCFA | 8.8±1.7 | 5.4±1.4 | 6.5±1.3 | 4.7±1.1 |
Abbreviations: M-SHIME, mucosal-simulator of human intestinal microbial ecosystem; SCFA, short-chain fatty acid.