Table 2. The singular value decomposition of the matrix containing abundances of 131 genus-like groups (as determined with the HITChip) of the human inocula, luminal and mucosal M-SHIME samples (n=5) resulted in six terms, which significantly explained the variation in the data set caused by either the location (=human inoculum, lumen M-SHIME or mucin layer M-SHIME) or source of the sample (=human donor A, B, C, D and E).

Term	P-value location	P-value source	% Variation explained	Cause of variation
1	0.0002	0.9492	39.6	Inoculum↔M-SHIME lumen/mucin layer
2	0.0000	0.9931	28.4	M-SHIME lumen↔M-SHIME mucin layer
3	0.8400	0.0008	8.4	Interindividual variability human inocula
4	0.9858	0.0205	5.6	Interindividual variability human inocula
5	0.6273	0.0371	4.5	Interindividual variability human inocula
6	0.9637	0.0242	3.1	Interindividual variability human inocula

Abbreviations: HITChip, human intestinal tract chip; M-SHIME, mucosal-simulator of human intestinal microbial ecosystem.