Table 1. Oligonucleotide probes designed in this study.
| Probe name | Specificity | No. target sequences/outgroup hits | Sequence (5′ to 3′) | FA % |
|---|---|---|---|---|
| bet I-B | Uncultured bet I-B tribe (Rhodoferax sp. relatives) | 3 | GAG TAG GCC GTT TCG TTC | 35 |
| bet I-C-449 | Uncultured bet I-C tribe (Curvibacter sp. relatives) | 7 | ATT AGC CAA GAC TTT TTC G | 20 |
| bet VI-994 | Uncultured bet VI tribe | 4 | GCA GAA TTC CGA ACA TGT | 35 |
| bet VI-H1 | Helper for bet VI-994 | GAG CAC TTC CGC ATC TCT | ||
| bet VI-H2 | Helper for bet VI-994 | CAA GGG TAG GTA AGG TTT | ||
| bet VI-H3 | Helper for bet VI-994 | CAC CTG TGT TCC GGT TC | ||
| GKS98-584 | Uncultured GKS98 (bet III) tribe | 72/2 | ACC TCT CTT TCC GAA CCG CC | 35 |
| GKS98-C | Competitor for GKS98-584 | AYC TYT CTT TCC GAA CCG CC | ||
| GKS98-H1 | Helper for GKS98-584 | TGC GCA CGC TTT ACG CCC | ||
| GKS98-H2 | Helper for GKS98-584 | TAA GCC CTG GGA TTT CAC | ||
| GKS98-H3 | Helper for GKS98-584 | CGC TTT CGT GCA TGA GCG |
Abbreviation: FA%, formamide concentration for stringent conditions.