Table 2. Summary of pyrosequencing and BLSOM classifications.
AVf | AVm | IOf | IOm | IPf | IPm | IRf | ATn | HFfmn | HLfmn | |
---|---|---|---|---|---|---|---|---|---|---|
Total no. of reads | 42 258 | 27 582 | 37 667 | 35 544 | 37 136 | 33 789 | 39 810 | 21 639 | 21 213 | 24 249 |
Average read length (nt) | 163.6 | 391.4 | 379.8 | 154.6 | 371.7 | 143.5 | 149.9 | 149.0 | 185.0 | 187.5 |
No. of long reads (≥300 bp) | 8160 | 22 201 | 29 804 | 2962 | 28 618 | 4210 | 5438 | 2906 | 4502 | 5186 |
No. of reads assigned to bacteria/archaea (%)a | 3951 (48.4) | 8389 (37.8) | 16 239 (54.5) | 1620 (54.7) | 18 711 (65.4) | 2468 (58.6) | 3782 (69.5) | 1222 (42.1) | 2946 (65.4) | 3750 (72.3) |
No. of reads assigned to a bacterial/archaeal phyla (%)b | 3841 (97.2) | 8272 (98.6) | 16 088 (99.1) | 1598 (98.6) | 18 556 (99.2) | 2439 (98.8) | 3748 (99.1) | 1213 (99.3) | 2925 (99.3) | 3728 (99.4) |
No. of phyla obtained | 27 | 28 | 28 | 23 | 28 | 25 | 22 | 27 | 24 | 20 |
No. of reads assigned to known bacterial/archaeal genera (%)c | 3419 (89.0) | 7068 (85.4) | 14 410 (89.6) | 1410 (88.2) | 16 566 (89.3) | 2131 (87.4) | 3398 (90.7) | 1119 (92.3) | 2605 (89.1) | 3319 (89.0) |
No. of reads assigned to candidate bacterial/archaeal genera (%) | 71 (1.8) | 62 (0.7) | 700 (4.4) | 66 (4.1) | 669 (3.6) | 98 (4.0) | 76 (2.0) | 13 (1.1) | 89 (3.0) | 75 (2.0) |
No. of bacterial/archaeal genera obtained | 158 | 223 | 215 | 143 | 185 | 162 | 133 | 201 | 138 | 156 |
Calculated using the formula: (No. of reads assigned to bacteria/archaea)/{No. of long reads (≥300 bp)} × 100.
Calculated using the formula: (No. of reads assigned to any phyla)/(No. of reads assigned to bacteria/archaea) × 100.
Calculated using the formula: (No. of reads assigned to any genera)/(No. of reads assigned to any phyla) × 100.