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. 2013 Mar 1;126(5):1176–1188. doi: 10.1242/jcs.116327

Fig. 6.

Fig. 6.

TCs involved in looping events have associated SNX4/Rab4 and Rab11. HMEC-1 cells were cotransfected with PAGFP–Rab4/mCherry–SNX4 (A) or mCherry–SNX4/PAGFP–Rab11 (B). PAGFP in individual sorting endosomes (SEs) was photoactivated. Scale bars: 1 µm. Green lines in A demarcate the plasma membrane. Images show selected frames from a dataset continuously acquired at a frame rate of 3 frames per second. (A) Selected frames from supplementary material Movie 7. A SNX4+Rab4+ TC extends from a sorting endosome (3–6.3 s) in the upper plane, segregates and subsequently moves to the lower plane at 24.6 s. This TC returns and merges with the same endosome (29.1 s) (shown schematically below). (B) Selected frames from supplementary material Movie 8. A SNX4+Rab11+ TC extends from a sorting endosome (0 s), segregates and subsequently returns and merges with the same endosome (137.1–182.4 s).