Fig. 2.

D1K2 phosphorylates Cdk2 substrates, increases growth rate, and sensitizes cells to paclitaxel. (A) Immunoblot analysis of Cdk2 substrates in the indicated cell lines. Actin served as a loading control. (B) Growth curves of the indicated cell lines after being treated with 0.1% DMSO or 1 nM paclitaxel for 72 hours. Cells were then reseeded in six-well plates and counted in triplicate each day for 1 week. (C) [³H]Thymidine incorporation of the indicated cell lines treated with increasing concentrations of paclitaxel for 72 hours. Values are the average of three replicate samples. Error bars represent the s.d. of three replicates. *P<0.05 and **P<0.005, using the unpaired t-test.