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. 2013 Mar 1;126(5):1247–1259. doi: 10.1242/jcs.120592

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© 2013. Published by The Company of Biologists Ltd

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Fig. 5. — PtdIns(4,5)P₂ depletion and retinal degeneration in trp mutants. (A) ERG recording from a trp mutant using a standard ERG set up. trp mutants show response inactivation to 200 ms dim test flashes (arrowheads) after prolonged illumination of 30 s (bar), reflecting PtdIns(4,5)P₂ depletion (Hardie et al., 2001). The response gradually recovered, representing regeneration of PtdIns(4,5)P₂. (B,C) PtdIns(4,5)P₂ depletion in trp mutants under degenerative condition. (B) Representative traces from a wild type, and a trp mutant before (dark-adapted) and ∼5 s after exposure to the red LED in a light box for 30 minutes. (Ci) ERG amplitude response to a test flash as a function of duration of exposure to red LED, normalized to dark-adapted response prior to exposure (V/V_max). 30 mins exposure caused near complete response inactivation (PtdIns(4,5)P₂ depletion) in trp mutants but not in wild-type flies. (Cii) Response recovery after 30 mins of red light exposure (n = 5, means ± s.e.m.). (D) Intensity dependence of response inactivation (V/V_max), reflecting PtdIns(4,5)P₂ depletion (i), and retinal degeneration (ii) in trp mutants. Severity of PtdIns(4,5)P₂ depletion (means ± s.e.m., n indicated in brackets) and retinal degeneration in trp (n≥7, means ± s.e.m.) had similar intensity dependence.