Fig. 3.

Cyclin D1 relocalized from the nucleus to the cytoplasm in 3D collagen culture. a Confocal images of cyclin D1 in MDA-MB-231 cells. Cyclin D1 staining was visualized with Alexa Fluor 488 conjugated secondary antibody (green). Nuclei were stained with DAPI (blue). Magnification 63X. b Western blots of phospho-Rb (Ser780) and Rb in 2D^P, 2D^CI and 3D^CI MDA-MB-231 cells. *p < 0.05 vs. 2D^P, ^ΔΔp < 0.01 vs. 2D^CI. c Western blot of c-Myc in 2D^P, 2D^CI and 3D^CI of MDA-MB-231 cells. *p < 0.05 vs. 2D^P, ^ΔΔp < 0.01 vs. 2D^CI. The relative quantity of phospho-Rb, total Rb or c-Myc protein was calculated as in Fig. 2b