Table 2.
Results of the triplicate analysis of the seven-protein mixture.
| MW (kDa) | Injection amount (pg) | Injection amount (fmole) | Peptide IDs# | Sequence coverage (%)# | |
|---|---|---|---|---|---|
| BSA | 69.5 | 440 | 6 | 3 | 6 |
| Cytochrome c | 11.7 | 36 | 3 | 16 | 73 |
| Myoglobin | 16.9 | 36 | 2 | 13 | 96 |
| α-casein | 24.5 | 60 | 2 | 2 | 20 |
| β-casein | 25.1 | 60 | 2 | 4 | 19 |
| Insulin chain b oxidized | 3.4 | 40 | 12 | 4 | 100 |
| β-lactoglobulin | 18.4 | 60 | 3 | 0 | 0 |
| Transthyretin | 15.7 | Un* | Un | 1 | 7 |
| Uncharacterized protein | 18.3 | Un | Un | 4 | 27 |
| BARX2 protein | 31.0 | Un | Un | 1 | 2 |
#
Peptide IDs and sequence coverage were from the combined triplicate runs.
*
The injection amounts of these proteins were unknown (Un).