Fig. 3.

Overexpression of P4HB enhances GBM cell survival against TMZ treatment. (A) Western blot shows increased P4HB expression in D54-S and U87-S cells after transfection with P4HB cDNA clone (D54-S-P4HB and U87-S-P4HB) from 48 h (48 h) to 120 h (120 h). (B) Cell viability assay shows increased P4HB expression can significantly enhance cell survival against TMZ treatment when compared to the wild-type (WT) parental (D54-S and U87-S) and vector control (D54-S-Vec and U87-S-Vec) GBM cells. *P = .05; **P = .01. (C) Table shows increased IC₅₀ in both D54-S-P4HB and U87-S-P4HB cells, compared with D54-S-WT and U87-S-WT, D54-S-Vec, and U87-S-Vec GBM cells.