| Sprague Dawley litter Embryonic Age | Volume per litter | CD1 litter Embryonic Age | Volume per litter |
| E13 | 30-50 μl | E10.5 | 15-20 μl |
| E14 | 40-75 μl | E12.5 | 15-20 μl |
| E16 | 50-90 μl | E14.5 | 10-15 μl |
| E18 | 40-75 μl | E16.5 | 5-10 μl |
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