Figure 4. Domain IV of TorA is not required for complex formation with TorD.

(A) TorD^His-containing fractions after metal chelate chromatography of cell extracts overproducing TorA_ΔCT and TorD^His were pooled, concentrated and applied to a HiLoad 16/60 Superdex 200 Prep Grade size-exclusion column. Eluted protein was monitored by measuring absorbance at 280 nm. MW, molecular mass. (B) SDS/PAGE analysis (12% gels) of the pooled fractions after metal chelate chromatography (Ni pool), and the non-concentrated peak fraction following gel-filtration chromatography. Molecular masses are indicated in kDa.