Figure 10.

pH sensitivity of complexes of DC-Chol/DOPE liposomes/pDNA and pH-sensitive PEGylated liposomes. The calcein release rate was calculated using the formula: (F1 − F₀)/(F₂ − F₀) × 100%. (A) pH-sensitive 1% PEGylated liposomes and complexes of DC-Chol/DOPE liposomes/pH-sensitive 1% PEGylated liposomes, (B) pH-sensitive 3% PEGylated liposomes and complexes of DC-Chol/DOPE liposomes/pH-sensitive 3% PEGylated liposomes, and (C) pH-sensitive 5% PEGylated liposomes and complexes of DC-Chol/DOPE liposomes/pH-sensitive 5% PEGylated liposomes.

Note: Data are presented as the mean ± standard deviation (n = 3).

Abbreviations: DC-Chol, 3β-[N-(N′,N′-dimethylaminoethane) carbamoyl] cholesterol; DOPE, dioleoylphosphatidyl ethanolamine; PEG, poly(ethylene glycol).