Confocal microscopy was performed to observe colocalization of FITC-labeled DC-Chol/DOPE (2/3) liposomes and rhodamine-labeled pH-sensitive PEGylated liposomes in complexes of DC-Chol/DOPE (2/3) liposomes/pH-sensitive PEGylated liposomes with a charge ratio of 1/1 (m/m [+/−]). (A) Rhodamine-labeled pH-sensitive PEGylated liposomes, (B) FITC-labeled DC-Chol/DOPE (2/3) liposomes, and (C) merged images.
Note: Bar represents 5 μm.
Abbreviations: DC-Chol, 3β-[N-(N′,N′-dimethylaminoethane) carbamoyl] cholesterol; DOPE, dioleoylphosphatidyl ethanolamine; FITC, fluorescein isothiocyanate; PEG, poly(ethylene glycol).