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. 2013 Apr 1;123(5):1939–1951. doi: 10.1172/JCI62185

Figure 5. Verification of the specificity of LNA–miR-mimic and LNA–miR-masks on KCNJ2/Kir2.1.

Figure 5

(A and B) Verification of the ability of the LNA–miR-mimic alone to reduce (A) and the LNA–miR-mask alone to increase (B) atrial Kir2.1 protein levels by Western blot on protein samples from atrial tissues. The respective negative control constructs were also examined. **P < 0.01 vs. WT; n = 6 /group. (C and D) Verification of the specificity of the LNA–miR-mimic to repress Kir2.1 without affecting the other untargeted genes, Cyclin D2 and Cyclin E2. (E and F) Verification of the specificity of the LNA–miR-masks to block action of miR-26 on Kir2.1 without altering its effects on 2 other proven target genes, Cyclin D2 and Cyclin E2 (27). miR-26a, adv–miR-26a; miR-masks, LNA–miR-masks; MM miR-mask, mismatched LNA–miR-mask. **P < 0.01 vs. WT; n = 6/group. Values are expressed as mean ± SEM.