Figure 5. Tg expression of Bcl-XL in 2C T cells rescues them from deletion in hosts with i.v. C1498.SIY cells.

(A) CFSE-labeled 2C or 2C^BCL-XL T cells were transferred into C57BL/6 mice. On day 1, mice received i.v. or s.c. C1498.SIY cells. On day 7, CFSE dilution of splenic 2C and 2C^BCL-XL T cells was analyzed. Representative CFSE dilution profiles are shown. (B) Absolute numbers of 2C T cells in spleens of mice in A. *P < 0.05. (C) 2C or 2C^BCL-XL T cells were transferred into mice and subsequently challenged with i.v. or s.c. C1498.SIY cells as in A. On day 7, spleen cells were restimulated with media or SIY peptide, and production of IFN-γ and TNF-α was analyzed. Numbers represent percent 2C T cells producing the indicated cytokines. (B and C) Data are representative of 2 experiments with 3 mice/group. (D) Percent 2C and 2C^BCL-XL T cells in spleens and livers of mice 24 days after i.v. C1498.SIY cell challenge. Representative plots are shown. Gated areas represent percent 2C or 2C^BCL-XL T cells among the entire CD8⁺ T cell population. Mean percent 2C and 2C^BCL-XL T cells in groups of 3 mice is also shown. *P < 0.05, 2C^BCL-XL versus 2C. Data are representative of 2 experiments.