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. 2013 Apr 24;123(5):1999–2010. doi: 10.1172/JCI63980

Figure 6. Agonistic CD40 ligation prevents T cell deletion, priming large numbers of activated T cells, in mice harboring C1498.SIY cells i.v.

Figure 6

(A) CFSE dilution of 2C T cells 7 days after transfer into C57BL/6 mice challenged with i.v. C1498.SIY cells and treated with anti-CD40 or isotype control Ab (IC). (B) Splenocytes from mice in A were restimulated with media or SIY peptide, and IFN-γ and TNF-α production by 2C T cells was assessed. Numbers represent percent cytokine-producing 2C T cells. (C) C57BL/6 mice received i.v. or s.c. C1498.SIY cells and were treated with anti-CD40 or isotype control Ab. On day 6, percent SIY-reactive splenic CD8+ T cells was analyzed. A negative control OVA tetramer was also used. *P < 0.05 versus all other groups. (D) IFN-γ ELISPOT analysis of splenocytes from mice in C. *P < 0.05 versus control Ab. (E) C57BL/6 mice received C1498.SIY cells i.v. on day –6 and were treated with anti-CD40 or isotype control Ab on days –6 and –3. On day 0, these mice were challenged with C1498.SIY cells s.c. Control mice received C1498.SIY cells i.v. or s.c. on day 0 only. IFN-γ ELISPOT analysis was performed on day 6. (F) C57BL/6 mice received C1498.SIY cells i.v. On days 0, 2, and 4, anti-CD40 or isotype control Ab was administered, and survival was assessed. *P = 0.002 versus control Ab. (G) C57BL/6 mice received i.v. C1498.SIY cells on day 0. On days 8, 10, 12, 17, 22, and 27, anti-CD40 or isotype control Ab was administered, and survival was assessed. *P = 0.05 versus control Ab. (H) C57BL/6 mice received FBL cells i.v. on day 0. On days 5, 7, 9, 13, and 18, anti-CD40 or isotype control Ab was administered, and survival was assessed. *P < 0.05 versus control Ab. Data are representative of 2 independent experiments with 3 (A–E) or 5 (F–H) mice/group.