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. 2013 Apr 1;123(5):2064–2077. doi: 10.1172/JCI64375

Figure 8. MKP-5 attenuates myoblast proliferation through a JNK/cyclin D3 pathway.

Figure 8

(A) SC-derived myoblasts isolated from pooled hind limb muscles were serum starved overnight and then restimulated with 20% FBS for the indicated times. Immunoblots show phosphorylation of MAPKs and expression of cyclin D3. CDK4 serves as a loading control. (B) Clonal proliferation assays were performed in which SC-derived myoblasts were treated with either JNK (SP600125) or p38 MAPK (SB203580) inhibitors for 6 days. (C) Cyclin D3 expression in response to either JNK or p38 MAPK inhibitors (5 mM). (A–C) Results represent at least 3 independent preparations from 8-week-old male mice. For each independent preparation, 2 mice from each genotype were used. Results represent the mean ± SEM. *P < 0.05 and **P < 0.01 compared with Mkp5+/+ mice. Symbols that differ (#, §, or ‡) indicate statistical significance between groups at P < 0.05.