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. 2013 Apr 8;123(5):2231–2243. doi: 10.1172/JCI64498

Figure 4. Pancreas-specific IKKα deficiency results in ER and oxidative stress.

Figure 4

(A–D) EM images showing dilated ER and autophagic vacuoles containing damaged ER in IkkαΔpan acinar cells. Scale bars: 500 nm. (E) qRT-PCR analysis of ER stress markers in IkkαF/F and IkkαΔpan pancreata. (F) Viability of IkkαF/F and IkkαΔpan acinar cells incubated with the indicated concentrations of tunicamycin for 24 hours. (G) Amylase release from IkkαF/F and IkkαΔpan acinar cells incubated with the indicated concentrations of cholecystokinin. (H) EM images showing swollen and fragmented mitochondria in IkkαΔpan pancreata. Scale bars: 500 nm. (I) qRT-PCR analysis of oxidative stress genes in IkkαF/F and IkkαΔpan pancreata. (J) Cis-aconitase activity in pancreata of 3-month-old IkkαF/F and IkkαΔpan mice. Results are shown as mean ± SEM. n = 3–5 mice per condition. *P < 0.05; **P < 0.01; ***P < 0.001.