Figure 2. Expression and cellular distributions of FZ1 and FZ8 receptor protein in rat SC after CCI.
(A) Western blot analysis showing time course for the expression of FZ1 and FZ8 proteins (n = 5). Representative bands are shown on the top; data summary is shown on the bottom. One-way ANOVA, *P < 0.05, **P < 0.01 versus sham. (B and C) Immunofluorescence showing expression and distribution of FZ1 and FZ8 (green) in the DH (B) and their colocalization with neuronal somata (NeuN, red), dendrites (MAP2, red), and astrocytes (GFAP, red), as well as microglial cells (IBA1 red) in the superficial DH ipsilateral to the CCI (C). Tissues were collected on day 1 for FZ1 and day 7 for FZ8 (B and C), respectively, after CCI. Original magnification, ×100 (B), ×200 (C), and ×400 (insert in C).
