Figure 2. CD148 deficiency does not attenuate inflammatory response following OVA allergen sensitization and challenge.

(A and D) BAL cell counts of total cells, macrophages, eosinophils, lymphocytes, and neutrophils in WT and CD148-deficient (Ptprj^TM–/TM–) mice following immunization and intranasal challenge with OVA or saline in C57BL/6 (A) or BALB/c (D) mice. (B and E) Histologic scoring on a scale of 0 to 4 by a blinded observer of H&E staining to quantify the degree of airway inflammation (left panel) and PAS staining to quantify mucus-producing goblet cells (right panel) in WT and Ptprj^TM–/TM– mice. (C and F) Relative OVA-specific serum IgE levels were measured by ELISA in WT and Ptprj^TM–/TM– mice. Data for all panels include 9–13 animals per group. Data show the mean ± SEM. *P < 0.05, unpaired 2-tailed Student’s t test. NS, nonsignificant.