BALB/c mice were immunized and sensitized with OVA, and cells from the lungs and mediastinal lymph nodes were stimulated with PMA and ionomycin and analyzed using intracellular cytokine staining. Total CD4 counts were similar in lung (A) and mediastinal lymph node (C). Absolute numbers of CD4+ IL4+ (B and D), CD4+ IL13+ (E and G), CD4+ IL17+ (F and H), CD4+ IFNγ+ (I and K), and γδTCR+ IL17+ (J and L) cells in both lung and lymph node were not statistically significantly different by unpaired 2-tailed Student’s t test. Data are the mean ± SEM for at least 5 mice per group.