Figure 4. CD148-deficient mice are protected from HDM-induced AHR with minimal attenuation of inflammatory response to allergen.

(A) Pulmonary resistance measurements in WT and Ptprj^TM–/TM– BALB/c mice following immunization and intranasal challenge with HDM. ***P < 0.001 for the WT HDM versus the Ptprj^TM–/TM– HDM group; ^###P < 0.001 comparing the highest dose of ACh in the WT saline group with the Ptprj^TM–/TM– saline group by 2-way ANOVA. (B) BAL cell counts of total cells, macrophages, eosinophils, lymphocytes, and neutrophils in WT and Ptprj^TM–/TM– mice. (C) Histologic scoring on a scale of 0 to 4 by a blinded observer of H&E staining to quantify degree of airway inflammation (left panel) and PAS staining to quantify mucus-producing goblet cells (right panel) in WT and Ptprj^TM–/TM– mice. (D) Total serum IgE levels (relative units) measured by ELISA in WT and Ptprj^TM–/TM– mice in the HDM model. Data for all panels include at least 10 animals per group. Data show the mean ± SEM. (B–D) *P < 0.05, **P < 0.01, unpaired 2-tailed Student’s t test.