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. 2013 Apr 8;123(5):2078–2093. doi: 10.1172/JCI66715

Figure 8. Knockdown of Hif1α and Hif2α reduces the hypoxia-induced invasion of cell lines derived from Pten;Braf melanomas.

Figure 8

(A) 2460 Pten;Braf melanoma cells were treated with the indicated doses of vemurafenib, PIK-90, or DMSO overnight. Whole-cell lysates were immunoblotted with the indicated antibodies. (B) Representative photomicrographs of 2130 and 2460 Pten;Braf melanoma cells, which have invaded through Matrigel chambers under hypoxia or normoxia. Original magnification, ×10. (C) Quantification of 2130 and 2460 Pten;Braf melanoma cells, which have invaded through Matrigel chambers. (D) 2130 and 2460 Pten;Braf melanoma cells were transfected with siRNAs against murine Hif1α or Hif2α and immunoblotted with the indicated antibodies. (E) Representative photomicrographs of 2130 and 2460 Pten;Braf melanoma cells that were transfected with siRNAs against murine Hif1α or Hif2α and allowed to invade through Matrigel chambers under hypoxia. Original magnification, ×10. (F) Quantification of 2130 and 2460 Pten;Braf melanoma cells that were transfected with siRNAs against murine Hif1α or Hif2α and allowed to invade through Matrigel chambers under hypoxia. Error bars show SEM. ***P < 0.0005;**P < 0.005; *P < 0.05.