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. 2013 Mar 13;33(11):4741–4753. doi: 10.1523/JNEUROSCI.2825-12.2013

Figure 2.

Figure 2.

Differences in [Mg2+]i-dependent modulation of gj in HeLa or N2A cells expressing Cx26, Cx32, Cx36, Cx43, Cx45, or Cx47; changes in [Ca2+]i are not involved in [Mg2+]i-dependent modulation of Cx36. All data represent mean gj (normalized to initial gj value). A, Normalized gj measured in HeLa Cx36-EGFP cell pairs using pipette solutions containing 10 mm free Mg2+ and 2 mm BAPTA (free Ca2+ = 25 nm) or 10 mm BAPTA (free Ca2+ ≈ 0). B, C, Normalized gj measured using pipette solutions containing 0.01 (B) or 5 mm free Mg2+ (C) in HeLa Cx36WT, HeLa Cx36-EGFP, or N2A Cx36-EGFP cell pairs. D, E, Normalized gj measured using pipette solutions containing 0.01 (D) or 5 mm (E) of free Mg2+ in HeLa cells expressing Cx26, Cx32, Cx36, Cx43, Cx45, or Cx47. Numbers of cell pairs are indicated within columns; *p < 0.05; ns, nonsignificant p values.