Figure 8.
Recovery of gj after decrease induced by Vj-gating or chemical-gating depends on [Mg2+]i. A, Bottom, Dynamics of gj recovery (normalized to initial gj value) after Vj steps (−80 mV; top) at [Mg2+]p = 5 mm. Small-amplitude repeated Vj ramps (±21 mV, 1.3 s; inset) were used to measure gj before and after Vj steps. Dashed line represents averaged decay in gj in the absence of Vj steps using pipette solutions with [Mg2+]p = 5 mm. B, Dynamics of gj recovery normalized to steady-state gj value (gj,ss) before decanol (0.5 mm) application to induce uncoupling at [Mg2+]p = 0.01 mm (Ba), 1 mm (Bb), and 5 mm (Bc). Small-amplitude repeated Vj ramps (same as in A) were used to measure gj. Gray dashed lines show levels of gj recovery during washout from decanol.