Divalent cations decrease gj of Cx36 GJs. A, Dynamics of gj (normalized to initial gj value) changes for different divalent cations. B, Average gj (normalized to initial gj values) from experiments using pipette solution containing the following: nominally zero divalents (n = 5); 2 mm free Mg2+ (n = 3), Ca2+ (n = 5), Ba2+ (n = 5), Mn2+ (n = 6), Cd2+ (n = 4), Zn2+ (n = 3); 0.2 mm free Zn2+ (n = 4). C, Average time for gj to undergo 95% of the change from initial to virtual steady-state conductance after beginning dual whole-cell voltage clamp.