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. 2012 Jun 13;14(1):19–24.

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Fig 1 — Results of nested MSPCR evaluation of DBC2 gene in blood samples in patients with breast cancer. Columns 2-9 (upper portion) represent the result of nested MSPCR of affected patients using unmethylated primers that did not show any bands. Columns 10 and 11 in the upper portion along with columns 1-5 in the lower portion belong to the same affected cases, using methylated primers. Column 7 is a 1000 bp ladder marker, column 1 is the negative control for unmethylated primers and column 12 is the negative control for methylated primers. We detected 23 methylation-specific bands in the blood samples of 50 affected women and 5 methylation-specific bands in the 30 normal control samples (p=0.007).