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Results of nested MSPCR analyses of the DBC2 gene in tumor tissue samples in affected patients. In column 1 (upper and lower portion) distilled water was used as a negative control. Columns 2-6 show the results of samples 40-44. The upper row belongs to the unmethylated primers and lower row to the methylated primers. In the 50 affected tissue samples, 17 had only methylated bands and the remainder (33 samples) had both methylated and un-methylated bands.
