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. 2012 Jun 13;14(1):53–60.

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Fig 3 — A. The negative control in situ RT-PCR with omission of primer showed no positive signal. B. The positive control in situ RT-PCR with no DNase treatment. The brownish color of the nucleus is very strong and intense. C. In situ RT-PCR detection of β-actin mRNA in breast tumour tissue of the CC group. A strong brown staining was observed in positive cells. D. In situ RT-PCR detection of β-actin mRNA in breast tumour tissue of the C250 group. A strong brown staining was observed in positive cells. E. In situ RT-PCR detection of β-actin mRNA in breast tumour tissue of the C500 group. A strong brown staining was observed in positive cells. F. In situ RT-PCR detection of β-actin mRNA in breast tumour tissue of the CT group. A strong brown staining was observed in positive cells. G. In situ RT-PCR detection of c-Myc mRNA in breast tumour tissue of the CC group. A strong brown staining was observed in positive cells. H. In situ RT-PCR detection of c-Myc mRNA in breast tumour tissue of the C250 group. A mild brown staining was observed in positive cells. I. In situ RT-PCR detection of c-Myc mRNA in breast tumour tissue of C500 group. A mild brown staining was observed in positive cells. J. In situ RT-PCR detection of c-Myc mRNA in breast tumour tissue of CT group. A mild brown staining was observed in positive cells. Magnification: x 200 for all slides.