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. Author manuscript; available in PMC: 2013 Apr 25.
Published in final edited form as: Nature. 2013 Feb 6;495(7439):98–102. doi: 10.1038/nature11847

Figure 2. NFIB loss enhances melanocyte stem-cell self-renewal and perturbs melanocyte stem cells activity in the hair follicle stem cell niche.

Figure 2

a, Ectopic pigmentation in telogen-phase cKO hair germ as detected by Fontana–Masson melanin staining and quantifications (n=3 mice per experiment, >40 hair follicles per mouse). b–e, Immunofluorescence and quantifications. b, c, Increased melanocytes and ectopic differentiation in telogen cKO hair follicles (30–50 hair follicles; 2 mice per experiment). KIT marks melanocyte stem cells and differentiated melanocytes; MITF andTYRP1 are differentiation-specific melanocyte markers. MCs, melanocytes. d, Precocious melanocyte differentiation begins in the niche at late catagen. Quantifications of TYRP1+ among KIT+ or Dct-EGFP+ melanocytes (>40 hair follicles; 2 mice per experiment). Analyses shown start at second telogen. SC, stem cell. e, Quantifications of melanocyte stem cells (TYRP1−) and differentiated melanocytes (TYRP1+) at different hair follicle stages (>30 hair follicles; 2 mice per experiment). f, Summary of data. McSC, melanocyte stemcell;MC, melanocyte. g, Lack of hair greying in ageing Nfib-cKO mice. Scale bars, 10 µm (a, b). *P<0.001. Error bars indicate s.e.m.