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. Author manuscript; available in PMC: 2013 Apr 25.
Published in final edited form as: Genesis. 2007 Feb;45(2):76–82. doi: 10.1002/dvg.20267

FIG. 5.

FIG. 5

In situ hybridization of E12.5 embryos. (a) Whole-mount lacZ staining of an E12.5 CCS-lacZ heart. Reproduced with permission from Rentschler et al. (2001). (b) Radioactive RNA in situ hybridization of E12.5 embryonic heart performed with a cocktail of three Slco3A1 antisense probes. (c). Hybridization of serial section with control Slco3A1 sense probes.(d) LacZ staining of an E12.5 minK-lacZ heart shows primarily subendocardial staining in the septum where the forming His bundle and bundle branches are located.(e) Slco3A1 expression does not appear to be in the endocardium (arrowheads).