. 2013 Apr 25;4:107. doi: 10.3389/fmicb.2013.00107

Table 2.

Primers and PCR conditions for MLSA.

Genes	Primer name	Primers (5′−3′)	Primer combination and annealing temperature (°C)	Amplicon size (bp)
atpA	atpA101F	ADGTDGGWGAYGGTGTYGC	101F-1081R: 55	980
	atpA1081R	CGTTRATAGCWGGTCTDATACC	101F-1081R: 55	980
	atpA983R	ACGTCACCBGCYTKWGTTTC	101F-983R: 52	882
dnaK	dnaK464F	GDCARGCWACMAARGAYGC	55	741
dnaK	dnaK1118R	GCMACWACYTCRTCDGG	55	741
gyrB	gyrB377F	CTYCAYGGWGTWGGWGC	52	1186
gyrB	gyrB1563R	TCWACRTCRGCRTCHGYCAT	52	1186
napA	napA181F	TTCTGTGGWACDGGWTGYGG	181F-1713R: 59	1532
	napA690F^*	ATGGCWGARATGCAYCC
	napA1714R	CKBGCHGCTTTRATCCARTG
	napA1347R^*	GRTTVAWWCCATTGTCCA
metG	metG145F	ACRGGWACMGATGARCATGG	55	686
metG	metG831R	GCDGGCCARTAWACDGYATG	55	686
tkt	tkt478F	AWSGSYGTRGGTATGGC	52	1063
	tkt1541R	TGKGTWGGDCCRTCYTC	52	1063

Primers used for sequencing reaction.