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Time course labeling of measured peptide fragments and calculated labeling of peptide-bound amino acid. Panel A demonstrates labeling profiles of QEVTDFAK and EVTDFAK fragments of QEVTDFAK peptide and calculated time-dependent labeling of glutamine (Q). Panel B demonstrates the labeling profiles of ADENAENCDK and DENAENCDK fragments of TCVDENAENCDK. The labeling of protein-bound alanine is estimated as a difference of the labeling of ADENAENCDK and DENAENCDK fragments.
